Composite

Part:BBa_K3052023:Design

Designed by: Ma WenHe   Group: iGEM19_XJTU-CHINA   (2019-10-13)


light controlling circuit - DHJ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 5648
    Illegal PstI site found at 5729
    Illegal PstI site found at 6152
    Illegal PstI site found at 6218
    Illegal PstI site found at 6308
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 6933
    Illegal NheI site found at 6956
    Illegal PstI site found at 5648
    Illegal PstI site found at 5729
    Illegal PstI site found at 6152
    Illegal PstI site found at 6218
    Illegal PstI site found at 6308
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2298
    Illegal BglII site found at 8493
    Illegal XhoI site found at 405
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 5648
    Illegal PstI site found at 5729
    Illegal PstI site found at 6152
    Illegal PstI site found at 6218
    Illegal PstI site found at 6308
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 5648
    Illegal PstI site found at 5729
    Illegal PstI site found at 6152
    Illegal PstI site found at 6218
    Illegal PstI site found at 6308
    Illegal NgoMIV site found at 5179
    Illegal NgoMIV site found at 8046
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression level of the two phases of this switch may be different since they are controlled by different promoters.


Source

This part comes from chemical synthesis.

References